[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 7, Number 3 (7-2017) ::
Iran J Ped Hematol Oncol 2017, 7(3): 171-179 Back to browse issues page
Evaluation of Blood Storage Lesions in Leuko-depleted Red Blood Cell Units
Dr Akbar Hashemi Tayer, Dr Naser Amirizadeh, Dr Mahtab Mghsodlu, Dr Mahin Nikogoftar, Dr Mohammad Reza Deyhim, Minoo Ahmadinejad
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
Abstract:   (77 Views)

Background: Red blood cells (RBCs) undergo biochemical and morphologic alterations during storage that are known as the storage lesions causing decreased RBC quality and are correlated with transfusion reactions in certain groups especially in infants and critically ill patients. Microvesicles (MVs) as one of storage lesions may be derived from various cell types and have key roles in several biological processes. The aim of the study was to evaluate various storage quality measures in RBC concentrates during storage under blood bank condition.

Materials and Methods: In this descriptive study, twenty leuko-depleted packed RBCs bags from healthy donors were prepared and stored at 4°C for up to 42 days. Samples were withdrawn at seven different times and evaluated for various hematological, biochemical, and hemolysis measures. In addition, red blood cell microvesicles (RBC-MVs) were separated and characterized based on the expression of Glycophorin A (Gly.A) antigen.

Results: The assessment of RBCs during cold storage showed significant increase in hemolysis rate, hematocrit (Hct), free hemoglobin, plasma potassium, and plasma lactate (p<0.0001), while a significant decrease in plasma sodium and glucose (p<0.0001) was observed. Significant increase was also identified in the RBC osmotic fragility (p<0.001). During the storage of RBCs in Saline- Adenine- Glucose- Mannitol (SAGM), the MVs count increased significantly. The majority of MVs had positive staining for Gly.A (74%) and it correlated with the changes in hemolysis rate (r= 0.77; p<0.001).

Conclusion: Storage of RBC was associated with important changes which influence biochemical parameters, hemolysis, and microvesiculation process that generally affected the product quality and may contribute to the negative post-transfusion outcome.

Keywords: Glycophorin, Leuko-depleted, Microvesicle, Red blood cell, Storage lesion
Full-Text [PDF 250 kb]   (30 Downloads)    
Type of Study: Research | Subject: Special
Received: 2017/01/21 | Accepted: 2017/04/13 | Published: 2017/07/16
Add your comments about this article
Your username or email:

Write the security code in the box >



XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hashemi Tayer A, Amirizadeh N, Mghsodlu M, Nikogoftar M, Deyhim M R, Ahmadinejad M. Evaluation of Blood Storage Lesions in Leuko-depleted Red Blood Cell Units. Iran J Ped Hematol Oncol. 2017; 7 (3) :171-179
URL: http://ijpho.ssu.ac.ir/article-1-326-en.html
Volume 7, Number 3 (7-2017) Back to browse issues page
Iranian Journal of Pediatric Hematology and Oncology
Persian site map - English site map - Created in 0.221 seconds with 832 queries by yektaweb 3444