TY - JOUR JF - SSU JO - Iran J Ped Hematol Oncol VL - 10 IS - 1 PY - 2020 Y1 - 2020/1/01 TI - Effect of Estradiol on miR-21& miR-155 Expression in promyelocytic leukemia-derived cell line NB4 TT - N2 - Background: Due to the estrogen participation in modulating the proliferation and commitment of stem cells and the effects of miR-21 and miR-155 expression on reduced proliferation and colony formation of acute myeloid leukemia (AML), the aim of the present study was to evaluate the effect of estradiol on expression of miR-21 and miR-155 in the NB4 cell line, as an acute promyelocytic leukemia (APL). Materials and Methods: In the present experiment, NB4 cells were treated with different quantities of estradiol (5, 25, 50, 75, 100, 150, 200, 250 μg/ml) and vehicle control for 24 and 48 hours. Viability, apoptosis, and cellular proliferation were estimated by trypan blue exclusion, flow cytometry, and MTT assays, respectively. The level of miR-155 and miR-21 expression was studied using absolute quantitative real-time PCR. Results: Results showed that estradiol in the effective dose (200 μg/ml) led to decreased cellular viability (in a dose dependent manner, P = 0.004) and apoptosis of NB4 cells. In addition, the expressions of miR-155 and miR-21 were significantly and dose-dependently decreased (p<0.05). Conclusion: Estradiol at the effective dose caused apoptosis in NB4 cell line. This substance can be used as a drug for the treatment of APL. However, further assessments are needed to support the effectiveness of estradiol in the treatment of APL. SP - 49 EP - 56 AU - Naghibzadeh, Robab AU - Obeidi, Narges AU - Farsinejd, Alireza AU - Khamisipour, Gholamreza AU - Tohid Far, Masoud AD - Department of Hematology, School of Para Medicine, Bushehr University of Medical Sciences, Bushehr, Iran KW - cute Myeloid Leukemia (AML) KW - Estradiol KW - MiR-155 KW - MiR-21 UR - http://ijpho.ssu.ac.ir/article-1-454-en.html DO - 10.18502/ijpho.v10i1.2169 ER -