Iranian journal of Pediatric Hematology and Oncology
Iranian journal of Pediatric Hematology and Oncology
Iran J Ped Hematol Oncol
Medical Sciences
http://ijpho.ssu.ac.ir
1
admin
2008-8892
2228-6993
8
7
14
8888
13
en
jalali
1391
3
1
gregorian
2012
6
1
2
2
online
1
fulltext
en
Efficacy of a New Autologus Platelet Gel: in Vitro Study
قلب
Heart
گزارش مورد
case report
Abstract
Background
Autologus platelet gel is easy to prepare and is relatively low cost. The aim of this study was to prepare and evaluate in vitro efficacy of autologus platelet gel.
Materials and Methods
In this experimental study, platelet concentrate and platelet poor plasma were prepared with aphaeresis method. Thrombin was prepared by mixing the plasma with calcium. Thrombin activity level was determined by spectrophotometric method. The platelet gel was obtained by adding thrombin and calcium to the platelet rich plasma. The concentration of growth factors was measured using the enzyme-linked immunosorbent assay technique.
Results
Platelet rich plasma contained a total average of 1×1011 platelet in the 100 ml product. The highest activity of prepared thrombin was achieved at a ratio of 5 vol (5ml) platelet poor plasma to 1 volium (1ml) calcium gluconate (p=0.02). Produced thrombin was stable for two hours at 24ºC, six hours at 4ºC, and more than three months at -20ºC (p=0.04). Concentration of platelet derived growth factor and transforming growth factors-β in platelet gel supernatants were generally high (23.2 ± 6 ng/ml, 18.2 ± 5ng/ml respectively), whereas epithelial growth factor and fibroblast growth factor were present at low concentration (870 ± 110 pg/ml, 24.7 ± 3 pg/ml respectively).
Conclusion
With proper production, large numbers of platelets in the platelet rich plasma will be activated, hence high concentration of growth factors will be produced.
In Vitro, Platelet-Derived Growth Factor, Platelet-Rich Plasma
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http://ijpho.ssu.ac.ir/browse.php?a_code=A-10-1-86&slc_lang=en&sid=1
A
Hashemi Tayer
Yes
Sh
Alizadeh
No
M
Heidari Bateni
No