Iranian journal of Pediatric Hematology and Oncology
Iranian journal of Pediatric Hematology and Oncology
Iran J Ped Hematol Oncol
Medical Sciences
http://ijpho.ssu.ac.ir
1
admin
2008-8892
2228-6993
8
7
14
8888
13
en
jalali
1395
12
1
gregorian
2017
3
1
7
2
online
1
fulltext
en
Promoter Methylation and Gene Expression in Human CD34+ Stem Cells Derived Erythroid Lineage by MicroRNA
قلب
Heart
پژوهشي
Research
<div>
<p><strong>Background: </strong>Stem Cell differentiation is a process composed of vast variety of factors which are controlled by a network of certain mechanisms. This study aims to determine the possible role of DNA methylation, a potent regulator of VHL, ECAD and RUNX3 genes during Erythroid differentiation driven by miR-451.</p>
<p><strong>Materials and Methods: </strong>To determine the methylation status of promoters and the expression levels of VHL, ECAD and RUNX3 genes, Methylation Specific PCR (MSP) and real-time PCR were used, respectively, on both Cord Blood CD34+ Hematopoietic Stem Cells and differentiated cells. To measure the expression levels of mir-451, mirna qpcr technique was used.</p>
<p><strong>Results: </strong>Our findings demonstrated a similar methylation pattern for the target genes before and after differentiation by miR-451. However, the expression levels were significantly increased after differentiation. Gene expression and surface marker analysis results further confirmed the potential of miR-451 for driving erythroid differentiation from hematopoietic stem cells.</p>
<p>Discussion: Our findings ruled out DNA methylation effect on the regulation of VHL, ECAD, and RUNX3 genes during miR-451 mediated erythroid differentiation. However, having CpG islands in their promoters, these three genes are candidates to be controlled by methylation which may not able to be detected by MSP method.</p>
<p><strong>Conclusion: </strong>Taken together in this study we have shown a successful erythroid differentiation mediated by miR-451 which is at least in part, independent of DNA methylation. Further understanding of the underlying mechanisms driven by eryhtroid differentiation may lead to therapeutic measures to alter disorders of hematopoietic stem cell differentiation.</p>
</div>
DNA methylation, Erythroid differentiation, Gene expression, MiR-451, Stem cell
105
116
http://ijpho.ssu.ac.ir/browse.php?a_code=A-10-70-122&slc_lang=en&sid=1
Mehdi
Azad
No
Department of Medical Laboratory Sciences, Faculty of Allied Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
Farshad
Forooghi
No
Department of Immunology, Qazvin University of Medical Sciences, Qazvin, Iran
Hassan
Ehteram
No
Department of Pathology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran
Mousa
Vatanmakan
No
Department of Hematology, Faculty of Allied Medicine, Tehran University of Medical sciences, Tehran, Iran
Hajar
Nasiri
No
Hematology-Oncology and Stem cell Transplantation Research Center, Tehran university of Medical Science, Tehran, Iran
Naser
Mobarra
Yes
Stem Cell Research Center, Department of Biochemistry, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.