Iranian journal of Pediatric Hematology and Oncology
Iranian journal of Pediatric Hematology and Oncology
Iran J Ped Hematol Oncol
Medical Sciences
http://ijpho.ssu.ac.ir
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admin
2008-8892
2228-6993
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8888
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jalali
1398
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gregorian
2020
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Use of Four-Color Flow Cytometric Assay for Discrimination of Hematogone from Lymphoblast: Critical Issue for MRD Assessment in B-ALL Patients
قلب
Heart
پژوهشي
Research
<div><strong>Background: </strong>Hematogones are normal B-cell precursor which can be seen in different physiological and pathological conditions. Due to variation in B-cell acute lymphoblastic leukemia (B-ALL) blasts immunophenotyping and interference of hematogones in minimal residual disease (MRD) assessment, precise discrimination of hematogones is very crucial. The purpose of this study was to evaluate the expression pattern of surface markers in hematogones and compare them with lymphoblasts.<br>
<strong>Material and Methods: </strong>In this applied study, flow cytometric analysis was performed using Coulter FC-500 and MXP software in 4-color combination and 6 different tubes. In this study, 85 patients diagnosed with acute lymphoblastic leukemia were evaluated. Out of these patients, 45 were boys and 40 were girls. Patients aged from 1 to 15 years old. In addition, 27 bone marrow samples from other patients aged 4 to 18 years were included in this investigation. These samples had been obtained for other diagnostic purposes, such as immune thrombocytopenic purpura and juvenile idiopathic arthritis.<br>
<strong>Results: </strong>During flow cytometric analysis, hematogones showed expressions of CD19, CD20, CD22, CD10, CD45, CD81, CD123, CD9, CD34 (partial expression), and tdt (partial expression). In these patients, hematgones were negative for CD66c expression. Lymphoblastic cells were positive for CD19, CD20 (in some cases), CD22, CD10, CD45, CD81, CD123, CD58, CD9, CD66c, CD34 (in most cases), and TDT. CD81 mean fluorescence intensity (MFI) in hematogones was higher than that in lymphoblasts. (112.5 (30-251) vs. 17.5 (5-30); P<0.0001)<br>
<strong>Conclusion: </strong>According to findings of this study, it seems that the use of CD81, CD58, CD123, CD66c, CD9, and CD81 MFI in combination with B-Cells associated markers can be very effective in differentiating hematogone from lymphoblast.</div>
B-ALL, Hematogone, Lymphoblast, MRD
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http://ijpho.ssu.ac.ir/browse.php?a_code=A-10-722-1&slc_lang=en&sid=1
Mehdi
Allahbakhshian Farsani
No
Department of Laboratory Hematology and Blood Banking, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Esmaeil
Shahabi Satlsar
0000-0001-5259-059X
No
Department of Laboratory Hematology and Blood Banking, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Alireza
Mohseni
Yes
Laboratory Hematology and Blood Banking, Faculty of Paramedical Sciences, Mazandaran University of Medical Sciences, Sari, Iran
Mazandaran University of Medical Sciences
Mohammad
Mosleh
No
Department of Laboratory Hematology and Blood Banking, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Shahid Beheshti University of Medical Sciences
Mahdieh
Mehrpouri
No
Department of Laboratory Hematology and Blood Banking, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Shahid Beheshti University of Medical Sciences
Mahnaz
Agaeipour
No
Takhte Tavous Pathobiology Laboratory, Flow Cytometry Department, Tehran, Iran
Takhte Tavous Pathobiology Laboratory
Mohammad Hossein
Mohammadi
No
Department of Laboratory Hematology and Blood Banking, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Shahid Beheshti University of Medical Sciences
Roohollah
Gholampour
No
Takhte Tavous Pathobiology Laboratory, Flow Cytometry Department, Tehran, Iran
Takhte Tavous Pathobiology Laboratory
Farzaneh
Jadali
No
Takhte Tavous Pathobiology Laboratory, Flow Cytometry Department, Tehran, Iran
Takhte Tavous Pathobiology Laboratory