Showing 7 results for Kalantar
Phd Mh Sheikhha , Phd Sm Kalantar , Phd N Ghasemi , Msc S Soleimanian ,
Volume 2, Issue 3 (9-2012)
Abstract
Abstract
Background
Polymorphism A1298C of the methylenetetrahydrofolate-reductase (MTHFR) gene has been implicated in spontaneous abortion. In this study, we determined the allele and genotype frequencies of this polymorphism in recurrent spontaneous abortion (RSA) and implantation failure after in vitro fertilization (IVF).
Materials and Methods
We performed a case–control study on 60 women with RSA and 72 women with implantation failure after IVF (both of the groups have a problem in embryo implantation, so each other compare to the health group) and 60 fertile women to investigate the association between MTHFR A1298G, and pregnancy loss by polymerase chain reaction restriction fragment length polymorphism (PCR-RLFP) technique.
Results
Among the RSA patients 29 (72.5%) were heterozygote and 7 (17.5%) of them were homozygote for MTHFR mutation. In addition, 46 (63.9%) of IVF failure patients were heterozygote and the frequency of homozygote was 17 (23.6%). While in the control group 28 (56.0%) were heterozygote but none of them were homozygote. So the mutation rate of MTHFR in patients with abortion was statistically different from that in controls. Also significant difference was found in the frequencies of MTHFR between the patients and IVF failure group (p <0.001).
Conclusion
Our study revealed that the genotypes of MTHFR A1298C were significantly associated with increased risk of implantation failure of abortion and IVF failure.
Dr A Hashemi , Dr Mh Sheikhha , Dr Ma Manouchehri , Dr Sm Kalantar ,
Volume 4, Issue 1 (3-2014)
Abstract
Background
Monosomy is defined as the presence of only one chromosome instead of two in humans. Partial monosomy occurs when only a portion of the chromosome is present in a single copy, while the rest has two copies. It can occur in unbalanced translocations or deletions.
Case report
In this report, a 6 years old girl was presented who was referred to the Pediatric Dep, Shahid Sadoughi Hospital,Yazd, Iran, due to multiple congenital anomalies such as: frontal bossing, horizontal palpebral fissure, small deepest eyes, aplastic nasal bridge, broad philtrum, low set ears, large prominent ears, short neck, microcephaly, pectus excavatum,
mental retardation, and dislocation of the hip.
In peripheral blood smear, platelets were decreased but other hematological levels were normal. The karyotype result indicated a mosaic monosomy and partial monosomy of chromosome 21.
Conclusion
According to this and other case reports of monosomy of chromosome 21, this disease had very low prevalence rate among live infants or children. The present case had some congenital anomalies that present with abnormal medical condition. Therefore these patients must be evaluated for chromosomal studies.
Dr S Hosseeini, Dr Sh Ansari, Dr E Kalantar, Dr M Sabzechian, Dr A Alibeik, Mr A Dorgalaleh,
Volume 4, Issue 2 (6-2014)
Abstract
Background
Autoimmune hemolytic anemia is a hematologic
disorder that is rarely observed in infants and young
children. Most of the cases are associated with viral
or bacterial infections. In some cases, AIHA can be
characterized by a chronic course and an
unsatisfactory control of hemolysis, thus requiring
prolonged immunosuppressive therapy.
Case report
Especially in children younger than 2 years of age,
the clinical course of the disease may show either
resistance to steroids or dependence on high-dose
steroids. We report here an infant fatal autoimmune
Conclusion
This case suggests that investigation for the presence
of CMV infection in infantile AIHA should be
considered. Severe hemolysis is rare but could be a
potentially life-threatening complication of CMV
infection described mostly in immune compromised
adults and children.
Mrs Fatemeh Eskandari , Mrs Hoda Pourkarim , Dr Amir H Pakpour , Dr Mehdi Goudarzi , Dr Naser Mobarra , Dr Mehdi Sahmani , Dr Ali Dehghanifard , Mrs Nasim Kalantari , Mr Khamisipour Gholamreza , Dr Mehdi Azad,
Volume 5, Issue 3 (8-2015)
Abstract
Background: VHL (von Hippel-Lindau), Runx-3 (Runt-related transcription factor 3), E-cadherin (Epithelial cadherin), P15 (INK4a, cyclin dependent kinase inhibitor), and P16 (INK4b) genes are essential in hematopoiesis. The aim of this study was to explore the correlation between gene expression and promoter methylation in CD34+ stem cells before and after differentiation to erythroid lineage.
Materials and Methods: CD34+ hematopoietic stem cells were separated from umbilical cord blood using MidiMacs (positive selection) system. Expanded CD34+ stem cells were differentiated into erythroid lineage with human recombinant erythropoietin (EPO). DNA extraction was done by QIAamp DNA Mini Kit. RNA was extracted using RNase Mini plus Kit. MSP (Methylation specific PCR) technique was done for methylation assay. Methylation status and expression assay was done for VHL, Runx-3, E-cadherin, P15, and P16 genes on both CD34+ stem cells and differentiated erythroid cells.
Results: The results showed that, before differentiation, P15 had comparative methylation pattern and average expression and it remained unchanged after differentiation (p=0.01). concerning P16, results revealed no methylation pattern and complete expression in absence of EPO and with EPO it changed to comparative status (p=0.01). E-cad and Runx-3 genes had relative methylation pattern and fully expression before and after differentiation but their expression after that, was increased and decreased Respectively (p=0.04). VHL gene had no significant methylation status before or after differentiation and its expression was complete (p=0.01).
Conclusion: The obtained results indicated that promoter methylation of P15, P16, VHL, Runx3 and E-cad was one of the definitive expression control mechanism of these genes.
Ms F Skandari, Mr A Allahverdi, Ms H Nasiri, Dr M Azad, Ms N Kalantari, Dr M Soleimani, Mr H Zare-Zardini,
Volume 5, Issue 4 (12-2015)
Abstract
Background
The aim of this study was the ex vivo expansion of Umbilical Cord Blood hematopoietic stem cells on biocompatible nanofiber scaffolds.
Materials and Methods
CD133+ hematopoietic stem cells were separated from umbilical cord blood using MidiMacs (positive selection) system by means of monocolonal antibody CD133 (microbeads) subsequently, flowcytometry method was done to assess the purity of separated cells. Isolated cells were cultured on plate (2 Dimensional) and fibronectin conjugated polyethersulfon nanofiber scaffold, simultaneously (3 Dimensional). Colony assay test was performed to show colonization ability of expanded cells.
Results
Cell count analysis revealed that expansion of hematopoietic stem cells in 2dimensional (2D) environment was greater than 3dimensional (3D) condition (p =0.01). Assessment of stem cell- phenotype after expansions was performed
by flowcytometric analysis which is showed that the maintenance of CD133 marker in expanded cells in 3 dimensional condition were higher than expanded cells in 2 dimensional condition (p=0.01). Moreover, colony assay test was performed before and after of expansion to show colonization ability of expanded cells both in 3D and 2D culture and results revealed more ability of 3D culture compared with 2D culture (p =0.03).
Conclusion
The results of current study confirmed that umbilical cord blood CD133+ haematopoietic stem cells are able to expand on fibronectin conjugated polyethersulfon scaffold. These findings indicated that 3D is a proper and valuable cell culture system for hematopoietic stem cells expansion, compared to 2D in invitro situation.
Mrs Fatemeh Eskandari, Mrs Hoda Pourkarim, Dr Mehdi Sahmani , Dr Mehdi Goudarzi, Dr Naser Mobarra, Dr Ali Dehghanifard , Dr Gholamreza Khamisipour , Mrs Nasim Kalantari, Mrs Hanieh Rahmani , Dr Mehdi Azad,
Volume 6, Issue 3 (9-2016)
Abstract
Background: VHL (von Hippel-Lindau), Runx-3 (Runt-related transcription factor 3), E-cadherin (Epithelial cadherin), P15 (INK4a, cyclin dependent kinase inhibitor), and P16 (INK4b) genes are essential in hematopoiesis. The aim of this study was to explore the correlation between gene expression and promoter methylation in CD34+ stem cells before and after differentiation to erythroid lineage.
Materials and Methods: CD34+ hematopoietic stem cells were separated from umbilical cord blood using MidiMacs (positive selection) system. Expanded CD34+ stem cells were differentiated into erythroid lineage with human recombinant erythropoietin (EPO). DNA extraction was done by QIAamp DNA Mini Kit. RNA was extracted using RNase Mini plus Kit. MSP (Methylation specific PCR) technique was done for methylation assay. Methylation status and expression assay was done for VHL, Runx-3, E-cadherin, P15, and P16 genes on both CD34+ stem cells and differentiated erythroid cells.
Results: The results showed that, before differentiation, P15 had comparative methylation pattern and average expression and it remained unchanged after differentiation (p=0.01). concerning P16, results revealed no methylation pattern and complete expression in absence of EPO and with EPO it changed to comparative status (p=0.01). E-cad and Runx-3 genes had relative methylation pattern and fully expression before and after differentiation but their expression after that, was increased and decreased Respectively (p=0.04). VHL gene had no significant methylation status before or after differentiation and its expression was complete (p=0.01).
Conclusion: The obtained results indicated that promoter methylation of P15, P16, VHL, Runx3 and E-cad was one of the definitive expression control mechanism of these genes.
Dr Ahmad Zare Bidaki, Dr Pouran Pourhakkak, Dr Fateme Montazeri, Dr Seyed Mehdi Kalantar, Dr Hoseini Hoseini, Dr Abolfazl Dehghanpour, Dr Majid Emtiazy,
Volume 13, Issue 3 (7-2023)
Abstract
Background: Gastrointestinal carcinoma comprises 5% of all pediatric cancer in children. Given that the possible and beneficial effect of the Jaft extract in the treatment of gastric cancer is not known and there is no comprehensive study in this regard, this study aimed to assess the effect of Jaft extract on gastric cancer cell lines.
Materials and Methods: In this case-control study, oak fruit was collected from the mountains of Lorestan province. A gastric cancer (AGS) cell line was obtained from the Institute Pasteur cell bank and was cultured. After the preparation of the ethanolic extract of Jaft, the cell viability of the gastric cancer cells treated with Jaft extract was investigated by MTT assay. Quantitative Reverse Transcription PCR was used for assessing the expression of BAX, and BCL2 genes.
Results: The half maximal inhibitory concentration (IC50) value of Jaft extract was 162 µg/ml. The BAX gene expression was different between the case and control groups. In this regard, the expression of the BAX gene was increased in the concentration of 162 (P<0.01) and 250 µg/ml (P<0.001) of Jaft extract compared to the control group. The BCL2 expression was different between the two groups (P<0.05). In this regard, the expression of the BCL2 gene was decreased in the concentration of 162 and 250 µg/ml of Jaft extract compared to the control group.
Conclusion: It was found that Jaft extract increased the apoptosis of gastric cancer cells; therefore, it seems that the hydroalchoholic extract of Jaft is an appropriate anticancer medication.
