Showing 4 results for Sharifi
Msc A Elikaei , Phd S M Hosseini , Phd Z Sharifi , Phd H Latifi , Msc H Nikbakht , Msc H Mirshafiee , Msc A Asadollahi ,
Volume 3, Issue 3 (9-2013)
Abstract
Abstract
Background
Despite improvement in safety of plasma transfusion some virus transmission still remains a problem. So as World Health Organization (WHO) recommends, many countries developed Pathogen Reduction Technologies (PRT) to inactivate pathogens, in plasma components. The Methylene Blue (MB) based methods is one of the most universal one. The purpose of this research was, produce a device that can inactivate viruses in MB environment.
Materials and Methods
In this interventional study, each Plasma Sample was illuminated by 70Pieces (PCs) of 1 w red Light Emitting Diodes (LEDs) from one side. These LEDs emit light at central wavelength of 627 nm with 20 nm Full Width at Half Maximum (FWHM). Two model viruses Herpes Simplex Virus (HSV) and Vesicular Stomatitis Virus (VSV) were used and Tissue Culture 50% Infection Dose (TCID50) was used to calculate virus
Log reduction. Two concentration of MB and 5 different illumination times were used.
Results
In 10 µm concentration of MB, HSV had 6.00±0.2 maximum log reduction that obtain after 60 minutes illumination and VSV had 5.50±0.3 maximum log reduction after 75 minutes illumination. In 1 µM concentration of MB, HSV had 5.20±0.3 maximum log reduction that obtain after 60 minutes illumination and VSV had 4.90±0.2 maximum log reduction after 75 minutes illumination.
Conclusion
Results of virus inactivation in this method were similar to other methods (P-value<0.05 in comparison with Spring method, and P-value>0.05 in comparison with Theraflex), and it showed this device could inactivate viruses according to WHO recommendation.
H Farhangi, N Sharifi, H Ahanchian, A Izanloo,
Volume 5, Issue 1 (3-2015)
Abstract
In this study, a 2.5-year-old boy suffering from a febrile seizure with normal laboratory tests and a history of immune hemolytic anemia was examined. Brain MRI demonstrated some tumors in the frontal, parietal, and occipital lobe that corroborated the pathology results of primary central nervous system lymphoma for the patient. The patient was treated with high-
dose of Methotrexate. Our result suggested regular and careful monitoring of patients with autoimmune hemolytic anemia in order to control the manifestations of concomitant disease such as lymphoma that may develop later
Roya Sharifi, Minoo Shahidi, Fatemeh Sadeghi Shirazi, Fereshteh Parhizkary, Mahmood Barati, Farhad Zaker, Kazem Mosavizadeh,
Volume 14, Issue 4 (10-2024)
Abstract
Background: A widespread crosstalk between inflammation and coagulation has been shown in numerous studies. This suggests that coagulation can trigger an inflammatory response which ultimately leads to coagulation activation. Previous research has shown that polyphenols can affect blood pressure and endothelial dysfunction, resulting in reduced risk of cardiovascular diseases. This study aimed to investigate whether Silymarin, a flavonolignans, could play a role in the interaction between inflammation and coagulation by influencing endothelial cells.
Materials and Methods: In this experimental study, human umbilical vein endothelial cells (HUVECs) were seeded with and without various concentrations of silymarin. In vivo, treatment with silymarin was also carried out. Coagulative and fibrinolytic factors, including Von Willebrand factor (VWF) and Factor VIII (FVIII), tissue plasminogen activator-1 (TPA-1), and inflammatory factors, including interleukin 8 (IL-8) and tumor necrosis factor-alpha (TNF-α), were evaluated by flow cytometry, Real-Time Polymerase Chain Reaction (qPCR), Enzyme-Linked Immunosorbent Assay (ELISA) and Immunocytochemistry (ICC).
Results: Silymarin increased the gene expression, release, and storage of VWF while diminishing the gene expression, release, and storage of TPA-1 (P ˂ 0.05). The activity of FVIII was dramatically increased, and IL-8 and TNF-α levels were augmented. The in vivo study also indicated an elevated plasma level of VWF and IL-8 by silymarin administration.
Conclusion: The results showed that, although silymarin reduces inflammatory factors, it can affect coagulation factors by increasing the levels of VWF and FVIII activity and inhibiting TPA-1 production, thereby making thrombosis probable. Consequently, it is advisable to prescribe this medication with caution for individuals who are susceptible to thrombotic events.
Dr Vahideh Takhviji, Dr Mostafa Jamali, Dr Mohammad Reza Deyhim, Dr Zohreh Sharifi,
Volume 15, Issue 2 (3-2025)
Abstract
Background: The limited shelf life of platelets has led to an increasing demand for longer-lasting products. This study aimed to develop a lyophilization protocol to preserve platelets by using trehalose, a desiccation-resistant sugar, and comparing its effectiveness to sucrose, a standard sugar for protein lyophilization.
Materials and Methods: In this interventional study, the platelets were loaded with trehalose (30, 60, or 100 mM) and 2% sucrose and then freeze-dried. Evaluations were performed of platelet count, aggregation responses to the agonists thrombin (1 U/ml), collagen (2 µg/ml), adenosine diphosphate (ADP) (20 µM), and the expression of the activation surface marker CD62P. Gamma radiation (30 and 40 kGy) was evaluated for pathogen inactivation in lyophilized products through measuring the reduction factors for viral titers (Herpes Simplex Virus-1 (HSV-1) and Poliovirus) and bacterial titers (S. epidermidis and E. coli). The analyses were conducted using SPSS v23.0.1 and GraphPad Prism v10.
Results: The platelet count in the 60 mM trehalose group showed no significant change after lyophilization (mean difference: 78.33; P = 0.31). The flow-cytometry analysis revealed a significant increase of CD62P in the control and sucrose-treated groups (P < 0.01 for all the groups), while trehalose significantly preserved the platelet function. This was demonstrated by lower CD62P (7.88%, P = 0.000) and higher thrombin-induced aggregation (53.46%, P = 0.01) and ADP-induced aggregation (29.8%, P = 0.001) compared to the other groups. Gamma radiation achieved a 5.2-log reduction at 30 kGy and a 6.2-log reduction at 40 kGy for Poliovirus, along with a consistent 7-log reduction for HSV-1 at both. Additionally, titers of S. epidermidis and E. coli were reduced by more than 7 logs, rendering them undetectable.
Conclusion: Lyophilized platelets stabilized with trehalose and sterilized with gamma radiation represent a promising approach for dealing with the current limitations in platelet storage and availability.
