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Showing 4 results for Epo
Infantile Fibrosarcoma in a Child: a Case Report
Md A Hashemi , Md S Tefagh , Md A Seifadini, Md M Moghimi ,
Volume 3, Issue 3 (9-2013)
Abstract
Abstract Background Infantile Fibrosarcoma is a rare soft tissue tumor in infants and children mostly located in extremities. An infantile and adult form has similar histopathological patterns but survival prognosis is much better in infantile form. Recurrence of infantile fibrosarcoma is common but the rates of metastasis are less than 10 percent in children younger than five Years and 50 percent in children more than 10 years old. Case presentation In this case report, we presented a nine years girl with a relapsing mass in her left hand. The pathologic findings showed sheets of spindle-shaped cells with suggested diagnose of infantile fibrosarcoma. She was successfully treated with combination of surgery and chemotherapy with a good outcome. Conclusion Infantile fibrosarcoma is a differential diagnose of soft tissue mass in infants and children. It has a good prognosis and distant metastasis is uncommon. Choice of treatment is surgery but chemotherapy and radiotherapy were useful in decrease metastasis.
Gene Expression and Promoter Methylation Status of VHL, Runx-3, E-cadherin, P15 and P16 Genes During EPO-Mediated Erythroid Differentiation of CD34+ Hematopoietic Stem Cells
Mrs Fatemeh Eskandari , Mrs Hoda Pourkarim , Dr Amir H Pakpour , Dr Mehdi Goudarzi , Dr Naser Mobarra , Dr Mehdi Sahmani , Dr Ali Dehghanifard , Mrs Nasim Kalantari , Mr Khamisipour Gholamreza , Dr Mehdi Azad,
Volume 5, Issue 3 (8-2015)
Abstract

Background: VHL (von Hippel-Lindau), Runx-3 (Runt-related transcription factor 3), E-cadherin (Epithelial cadherin), P15 (INK4a, cyclin dependent kinase inhibitor), and P16 (INK4b) genes are essential in hematopoiesis. The aim of this study was to explore the correlation between gene expression and promoter methylation in CD34+ stem cells before and after differentiation to erythroid lineage.

Materials and Methods: CD34+ hematopoietic stem cells were separated from umbilical cord blood using MidiMacs (positive selection) system. Expanded CD34+ stem cells were differentiated into erythroid lineage with human recombinant erythropoietin (EPO). DNA extraction was done by QIAamp DNA Mini Kit. RNA was extracted using RNase Mini plus Kit. MSP (Methylation specific PCR) technique was done for methylation assay. Methylation status and expression assay was done for VHL, Runx-3, E-cadherin, P15, and P16 genes  on both CD34+ stem cells and differentiated erythroid cells.

Results: The results showed that, before differentiation, P15 had comparative methylation pattern and average expression and it remained unchanged after differentiation (p=0.01). concerning P16, results revealed no methylation pattern and complete expression in absence of EPO and with EPO it changed to comparative status (p=0.01). E-cad and Runx-3 genes had relative methylation pattern and fully expression before and after differentiation but their expression after that, was increased and decreased  Respectively (p=0.04). VHL gene had no significant methylation status before or after differentiation and its expression was complete (p=0.01).

Conclusion: The obtained results indicated that promoter methylation of P15, P16, VHL, Runx3 and E-cad was one of the definitive expression control mechanism of these genes.


Gene Expression and Promoter Methylation Status of VHL, Runx-3, E-cadherin, P15 and P16 Genes During EPO-Mediated Erythroid Differentiation of CD34+ Hematopoietic Stem Cells
Mrs Fatemeh Eskandari, Mrs Hoda Pourkarim, Dr Mehdi Sahmani , Dr Mehdi Goudarzi, Dr Naser Mobarra, Dr Ali Dehghanifard , Dr Gholamreza Khamisipour , Mrs Nasim Kalantari, Mrs Hanieh Rahmani , Dr Mehdi Azad,
Volume 6, Issue 3 (9-2016)
Abstract

Background: VHL (von Hippel-Lindau), Runx-3 (Runt-related transcription factor 3), E-cadherin (Epithelial cadherin), P15 (INK4a, cyclin dependent kinase inhibitor), and P16 (INK4b) genes are essential in hematopoiesis. The aim of this study was to explore the correlation between gene expression and promoter methylation in CD34+ stem cells before and after differentiation to erythroid lineage.

Materials and Methods: CD34+ hematopoietic stem cells were separated from umbilical cord blood using MidiMacs (positive selection) system. Expanded CD34+ stem cells were differentiated into erythroid lineage with human recombinant erythropoietin (EPO). DNA extraction was done by QIAamp DNA Mini Kit. RNA was extracted using RNase Mini plus Kit. MSP (Methylation specific PCR) technique was done for methylation assay. Methylation status and expression assay was done for VHL, Runx-3, E-cadherin, P15, and P16 genes  on both CD34+ stem cells and differentiated erythroid cells.

Results: The results showed that, before differentiation, P15 had comparative methylation pattern and average expression and it remained unchanged after differentiation (p=0.01). concerning P16, results revealed no methylation pattern and complete expression in absence of EPO and with EPO it changed to comparative status (p=0.01). E-cad and Runx-3 genes had relative methylation pattern and fully expression before and after differentiation but their expression after that, was increased and decreased  Respectively (p=0.04). VHL gene had no significant methylation status before or after differentiation and its expression was complete (p=0.01).

Conclusion: The obtained results indicated that promoter methylation of P15, P16, VHL, Runx3 and E-cad was one of the definitive expression control mechanism of these genes.


Investigating the Effect of Senior Managers' Compliance in Reporting Nurses' Treatment Errors in Pediatric Wards
Mr Hossein Nazmieh , Mrs Maryam Soroush , Mrs Farahnaz Farnia , Mrs Zarrin Habibian , Mrs Ashraf Sadat Dehghan , Dr Mehrdad Roozbeh,
Volume 8, Issue 3 (5-2018)
Abstract
Background: The occurrence of medical errors in therapeutic centers is important due to its critical nature in terms of health, patient safety, and notable clinical and economic outcomes. One of the solutions to manage this problem in the field of nursing is error reporting and recording. Error reporting, on one hand, improves patient care quality and safety and; on the other hand, provides valuable information to prevent future errors. Therefore, considering the importance of error reporting, the aim of this study was to determine the effect of senior managers' compliance in reporting nurses' treatment error in pediatric ward of Shahid Sadoughi Hospital in Yazd.
Materials and Methods: This interventional study included all nurses working in pediatric wards. The intervention was defined as various safety management drivers and encouragement of staff to report errors without any fears or concerns from senior managers. The error reports was recorded and comprised before and after intervention. For daat anlysis, SPSS (version 21) was run.
Results: Following the intervention, over the course of a year, a total of 327 errors were reported. With respect to wards, 36.9% of errors occurred in pediatric oncology ward, 40% in PICU, 25.6% in pediatrics, 8% in emergency department of pediatrics, and 15.9% in NICU. However, only 32 errors were reported during the last year. Data analysis indicated a significant increase in error reporting following the intervention (P-value = 0.021). Furthermore, the results showed that mostly errors occurred in morning shift. Considering the error type, medication error was the most frequent; and considering the reason, non-compliment with the principles of drug adminstration got the highest frequency.
Conclusion: The most important step in reducing errors is to eliminate the obstacles against reporting errors by creating a situation in which each nursing staff can honestly report his/her error. Therefore, regarding a significant difference before and after the intervention, it is recommended that senior managers consider medical treatment error reporting as their priorities.

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