Iranian journal of Pediatric Hematology and Oncology

en Parvovirus B19 Infection May Potentially Determine the Fate of Hematopoiesis by Altering the Human Bone Marrow Mesenchymal Stem Cells Differentiation عمومى General پژوهشي Research <div style="border:double windowtext 1.5pt; padding:1.0pt 4.0pt 1.0pt 4.0pt">Background: Human bone marrow mesenchymal stem cells (hBM-MSCs), as supporters for hematopoiesis, differentiate into osteoblasts and adipocytes. Studies showed that infection of hBM-MSCs by Parvovirus B19 (B19V) can affect the differentiation capability of hBM-MSCs. This study aims to evaluate B19V effects on the differentiation of hBM-MSCs. Materials and Methods: In this experimental study hBM-MSCs were cultured up to passage 3. Nucleofection was subsequently employed to deliver a plasmid containing the B19V genome into the cells. The transfected cells were then differentiated into osteoblast and adipocyte lineages. qRT-PCR was then performed to analyze the differentiation 14 days after transfection. Results: On the 14th day after induction the findings demonstrated a significant increase in adipocyte-specific (PPARγ and LPL) gene expression compared to the control group (p<0.05) and a slight but not statistically significant decrease in the expression of the osteocyte-specific genes (RUNX2 and osteocalcin) (p>0.05). Conclusion: The results suggest that B19V infection can promote the differentiation of hBM-MSCs towards adipocytes and affect the bone marrow microenvironment as well as hematopoiesis.</div> Adipocyte, Bone marrow, Mesenchymal stem cell, Osteoblast, Parvovirus B19 241 250 http://ijpho.ssu.ac.ir/browse.php?a_code=A-10-911-1&slc_lang=en&sid=1 Azin Elmi amir9226012@gmail.com 0000-0003-3345-0609 No Department of Medical Biotechnology, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran Department of Medical Biotechnology, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran Amir Atashi Dnagen.bc@gmail.com 0000-0002-0885-4065 No Stem Cells and Tissue Engineering Research Center, Shahroud University of Medical Sciences, Shahroud, Iran. Stem Cells and Tissue Engineering Research Center, Shahroud University of Medical Sciences, Shahroud, Iran. Nematollah Gheibi gheibi_n@yahoo.com 0000-0001-7503-0894 No Department of Medical Biotechnology, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran. Department of Medical Biotechnology, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran. Shahin Amiri shaahinamiri@gmail.com 0000-0003-0596-2076 No Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Monireh Ajami monirah1253@gmail.com 0000-0002-2705-8259 No Department of Hematology, Faculty of Paramedical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran Department of Hematology, Faculty of Paramedical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran Mansoureh Ajami Ajami12@gmail.com Ajami: https://orcid.org/0000-0003-1977-0753 No Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Shahroud University of Medical Sciences, Shahroud, Iran Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Shahroud University of Medical Sciences, Shahroud, Iran Razieh Mohammadihaji Mohammadihaji@gmail.com 0000-0002-0384-2268 No Department of Medical Biotechnology, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran. Department of Medical Biotechnology, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran. Naeimeh Khodabandeloo 0009-0004-9216-8798 No Department of Medical Laboratory Sciences, Faculty of Allied Medicine, Qazvin University of Medical Sciences, Qazvin, Iran. Department of Medical Laboratory Sciences, Faculty of Allied Medicine, Qazvin University of Medical Sciences, Qazvin, Iran. Mehdi Azad maali.amirhosein@gmail.com 0000-0000-0000-0000 Yes Department of Medical Laboratory Sciences, Faculty of Allied Medicine, Qazvin University of Medical Sciences, Qazvin, Iran. Department of Medical Laboratory Sciences, Faculty of Allied Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.