Iranian journal of Pediatric Hematology and Oncology

en Evaluating the expression of LKB1, SHMT1, and GLDC genes in Acute Lymphoblastic Leukemia patients هماتولوژی Hematology پژوهشي Research <div style="border:double windowtext 1.5pt; padding:1.0pt 4.0pt 1.0pt 4.0pt">Background: Epigenetic changes in cancer cells have an immense effect on tumorigenesis. As a tumor suppressor and an epigenetic regulator, liver kinase B1 (LKB1) reduces gene methylation by downregulating metabolic pathways such as the serine-glycine pathway. This study seeks to examine the gene expression levels of serine hydroxymethyltransferase 1 (SHMT1) and glycine decarboxylase (GLDC), as two serine-glycine pathway regulatory genes, along with LKB1 in acute lymphoblastic leukemia (ALL) patients. Materials and Methods: In this analytical study, qRT-PCR was used to evaluate the gene expression levels of LKB1, SHMT1, and GLDC in 50 ALL patients with an average age of 11.64 ± 10.6 years. The patients were compared to 10 healthy controls. Subsequently, the correlation between the gene expression levels and the patients’ demographic data was investigated. Results: No significant difference was found between the ALL patients and the control individuals in terms of LKB1 and GLDC gene expressions, but SHMT1 was significantly overexpressed in the ALL patients (p = 0.003). Moreover, there was a significant association between GLDC and the other SHMT1 (p = 0.020) and LKB1 (p = 0.047). No significant connection was also found between the age (pL = 0.304, pS = 0.305, pG = 0.899), gender (pL = 0.475, pS = 0.299, pG = 0.388), and blast percentage (pL = 0.335, pS = 0.148, pG = 0.459) of the patients and the genes. Conclusion: The increased expression of SHMT1 suggests the oncogenic role of this gene. Thus, the present study offers a novel diagnostic marker in ALL patients.</div> Glycine dehydrogenase , Precursor cell lymphoblastic, leukemia-Lymphoma, STK11 protein 217 226 http://ijpho.ssu.ac.ir/browse.php?a_code=A-10-1097-1&slc_lang=en&sid=1 Pegah Farajzadeh p.fjz1995@gmail.com 0009-0004-6845-6570 No Department of Genetics, Faculty of Advanced science and Technology, Tehran Medical sciences, Islamic Azad university, Tehran, Iran Department of Genetics, Faculty of Advanced science and Technology, Tehran Medical sciences, Islamic Azad university, Tehran, Iran Flora Forouzesh f8forouzesh@gmail.com 0000-0002-2747-3479 No Assistant professor, Department of Genetics, Faculty of Advanced science and Technology, Tehran Medical sciences, Islamic Azad university, Tehran, Iran Assistant professor, Department of Genetics, Faculty of Advanced science and Technology, Tehran Medical sciences, Islamic Azad university, Tehran, Iran Maryam Nabigol maryamnabygol@gmail.com 0009-0006-5374-7177 No Department of Hematology and Blood Bank, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran Department of Hematology and Blood Bank, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mehdi Allahbakhshian Farsani Mbakhshian1360@gmail.com 0000-0002-3910-0119 Yes Department of Hematology and Blood Bank, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran Department of Hematology and Blood Bank, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran