Iranian journal of Pediatric Hematology and Oncology

en In Silico Identification of Effective Genes for Acute Leukemia Classification Using a Spline Regression-based Framework عمومى General پژوهشي Research <div style="border:double windowtext 1.5pt; padding:1.0pt 4.0pt 1.0pt 4.0pt">Background: Microarray technology enables the examination of gene expression in thousands of genes and can be highly effective in identifying various types of cancers, including leukemia. However, many genes in microarray data are redundant and lack useful information for cancer diagnosis. The main objective of this study is to identify relevant and effective genes in classification of leukemia microarray data using a spline regression-based method, taking into account the correlation between genes. Materials and Methods: In this analytical study, leukemia microarray data are used to identify relevant genes in classification of leukemia into Acute Myeloid Leukemia (AML) and Acute Lymphoblastic Leukemia (ALL) using a spline regression-based gene selection method, called SRS3FS based on ℓ2,p-norm (0 Results: In this study, the classification results of SVM algorithm for 5, 10, 15, and 20 genes reveal that the SRS3FS method, employing ℓ2,1/4-norm, ℓ2,1/2-norm and ℓ2,3/4-norm, exhibited the highest accuracy of 97.06% when identifying 10 genes for distinguishing between AML and ALL. Moreover, the leukemia data was classified into AML and ALL with an accuracy of 100%, using a gene identified by the SRS3FS method based on ℓ2,3/4-norm and ℓ2,1-norm. The gene labeled as number 3252, annotated as GLUTATHIONE S-TRANSFERASE, MICROSOMAL, is recognized as the most important gene. Conclusion: The experimental results on leukemia microarray data demonstrate that the spline regression-based gene selection method can effectively identify relevant genes in classification and prediction of leukemia.</div> Acute lymphocytic leukemia, Acute myeloid leukemia, Gene expression, Sparse gene selection, Spline regression 104 115 http://ijpho.ssu.ac.ir/browse.php?a_code=A-10-822-4&slc_lang=en&sid=1 Maryam Yazdanparast M.Yazdanparast@gmail.com No Department of pediatrics, Shahid Sadoughi hospital, Shahid Sadoughi University, Yazd, Iran Department of pediatrics, Shahid Sadoughi hospital, Shahid Sadoughi University, Yazd, Iran Razieh Sheikhpour rsheikhpour@ardakan.ac.ir 0000-0002-3119-3349 Yes Department of Computer Engineering, Faculty of Engineering, Ardakan University, P.O. Box 184, Ardakan, Iran Department of Computer Engineering, Faculty of Engineering, Ardakan University, P.O. Box 184, Ardakan, Iran Morteza Zangeneh Soroush No Department of Biomedical Engineering, Science and Research branch, Islamic Azad University, Tehran, Iran Department of Biomedical Engineering, Science and Research branch, Islamic Azad University, Tehran, Iran Fatemeh Ghanizadeh Fatemeh.Ghanizadeh@gmail.com No Hematology and Oncology research center, Shahid Sadoughi University of Medical sciences, Yazd, Iran Hematology and Oncology research center, Shahid Sadoughi University of Medical sciences, Yazd, Iran