Iranian journal of Pediatric Hematology and Oncology
Iranian journal of Pediatric Hematology and Oncology
Iran J Ped Hematol Oncol
Medical Sciences
http://ijpho.ssu.ac.ir
1
admin
2008-8892
2228-6993
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8888
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jalali
1403
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gregorian
2024
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online
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Evaluating the Role of Silymarin in Coordinating the Relationship between Inflammation and Thrombosis by Affecting Endothelial Cells
سلولی-مولکولی
Cellular-molecular
پژوهشي
Research
<div style="border: 1.5pt double windowtext; padding: 1pt 4pt; text-align: justify;"><span style="font-family:Tahoma;"><span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.0pt">Background: </span></b><span style="font-size:10.0pt">A widespread crosstalk between inflammation and coagulation has been shown in numerous studies. This suggests that coagulation can trigger an inflammatory response which ultimately leads to coagulation activation. Previous research has shown that polyphenols can affect blood pressure and endothelial dysfunction, resulting in reduced risk of cardiovascular diseases. This study aimed to investigate whether Silymarin, a flavonolignans, could play a role in the interaction between inflammation and coagulation by influencing endothelial cells.</span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.0pt">Materials and Methods: </span></b><span style="font-size:10.0pt">In this experimental study<b>,</b> human umbilical vein endothelial cells (HUVECs) were seeded with and without various concentrations of silymarin. In vivo, treatment with silymarin was also carried out. Coagulative and fibrinolytic factors, including Von Willebrand factor (VWF) and Factor VIII (FVIII), tissue plasminogen activator-1 (TPA-1), and inflammatory factors, including interleukin 8 (IL-8) and tumor necrosis factor-alpha (TNF-α), were evaluated by flow cytometry, Real-Time Polymerase Chain Reaction (qPCR), Enzyme-Linked Immunosorbent Assay (ELISA) and Immunocytochemistry (ICC).</span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.0pt">Results: </span></b><span style="font-size:10.0pt">Silymarin increased the gene expression, release, and storage of VWF while diminishing the gene expression, release, and storage of TPA-1 (P ˂ 0.05). The activity of FVIII was dramatically increased, and IL-8 and TNF-α levels were augmented. The in vivo study also indicated an elevated plasma level of VWF and IL-8 by silymarin administration.</span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.0pt">Conclusion: </span></b><span style="font-size:10.0pt">The results showed that, although silymarin reduces inflammatory factors, it can affect coagulation factors by increasing the levels of VWF and FVIII activity and inhibiting TPA-1 production, thereby making thrombosis probable. Consequently, it is advisable to prescribe this medication with caution for individuals who are susceptible to thrombotic events.</span></span></span></span></div>
Factor VIII (FVIII), Inflammation, Silymarin, Von Willebrand factor (VWF)
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299
http://ijpho.ssu.ac.ir/browse.php?a_code=A-10-1105-1&slc_lang=en&sid=1
Roya
Sharifi
roya.sharifi071@gmail.com