Iranian journal of Pediatric Hematology and Oncology
Iranian journal of Pediatric Hematology and Oncology
Iran J Ped Hematol Oncol
Medical Sciences
http://ijpho.ssu.ac.ir
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admin
2008-8892
2228-6993
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8888
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jalali
1403
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gregorian
2025
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fulltext
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The Gene Expression Levels of ETS2, ADAM28, and GPRC5D Genes in Acute Lymphoblastic Leukemia Patients
انکولوژی
Oncology
پژوهشي
Research
<div style="border: 1.5pt double windowtext; padding: 1pt 4pt; text-align: justify;"><span style="font-family:Tahoma;"><span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.0pt">Background: </span></b><span style="font-size:10.0pt">Genetic biomarkers significantly influence the pathological differentiation and proliferation of lymphoid precursor cells, contributing to the development of Acute Lymphoblastic Leukemia (ALL) in children. This case-control study aimed to assess the expression levels of three potential biomarkers: <i>ETS2, ADAM28</i>, and <i>GPRC5D</i>, in patients with ALL.</span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.0pt">Materials and Methods:</span></b><span style="font-size:10.0pt"> In this cross-sectional study, a group of ALL patients (n=65) referred to the hematology-oncology departments of Nemazee Hospital and Amir Hospital in Shiraz, Iran, from May 2018 to May 2021 were selected as the patient group. A control group (n=65) of age- and gender-matched volunteers was also selected.</span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><b> </b><span style="font-size:10.0pt">Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to study the expression profiles of these genes in patients and compare the results to those of a control group. The study included 65 patients with ALL and 65 healthy participants. The Pfaffl method of relative quantification, which compares the threshold cycle of a constitutive gene (<i>TBP</i>) with the test gene of each sample in duplicate, was used to determine the relative levels of <i>ETS2</i>, <i>ADAM28</i>, and <i>GPRC5D </i>gene expression. SPSS software version 16 was used for statistical analysis. Nonparametric tests were used to analyze non-normally distributed data, and the Mann-Whitney test was used to compare the medians and ranges and a p-value of 0.05 was considered significant.</span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.0pt">Results: </span></b><span style="font-size:10.0pt">The patient group showed significantly greater expression of the <i>ETS2</i> and <i>ADAM28</i> genes compared to the control group. (P <0.0001; fold changes of 2.80 and 2.14, respectively), while <i>GPRC5D</i> expression remained unchanged (P >0.05).</span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.0pt">Conclusion: </span></b><span style="font-size:10.0pt">These genetic biomarkers in patients with ALL may play an oncogenic role in the pathogenesis of the disease and could serve as potential novel biomarkers for ALL.</span></span></span></span></div>
Acute Lymphoblastic Leukemia, ADAM28, Biomarker, ETS2, Pathogenesis
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http://ijpho.ssu.ac.ir/browse.php?a_code=A-10-1140-1&slc_lang=en&sid=1
Farnoush
Farokhian
farokhian.f@yahoo.com