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:: Volume 5, Number 3 (8-2015) ::
Iran J Ped Hematol Oncol 2015, 5(3): 155-160 Back to browse issues page
The roles of IL-2, IL-7, and IL15 Ligands in B Cells development
MS Z Aliyari, MS F Alami, Ms T Mostafavi, Dr H Taiefi Nasrabadi, Dr J Soleimanirad, Dr H Nozad Charoudeh
Abstract:   (861 Views)
Abstract Background B cells play an important role in the immune system due to production of the immunoglobulin and secreting several cytokines. It has been shown that B cells produced successfully in the presence of stem cell factor (SCF) and Flt3 ligand (Flt3L). IL2, IL7, and IL15 cytokines as  -common family have an essential cytokines may support either synergistically or in an additive manner to B cell proliferative activity. Thus, the present study focused specifically on IL2, IL7, and IL15 cytokines that may play a critical role in B cell proliferation in cord blood. In this study, we evaluated the generation of B cells from CD34+/- cord blood mononuclear cells by using IL-2, IL- 7, and IL-15. Material and Methods Cord blood mononuclear cells were cultured for 21 days in presence of different combination of IL-2, IL-7, and IL-15. Harvested cells were analyzed by flow cytometry at days 0, 7, 14 and, 21. Results Present study showed that B cell differentiation from CD34+ cord blood mononuclear cells was increased by using IL-2 and IL-7 at different time points however, IL7 was more effective (P value < 0.0001). In contrast, IL-15 didn’t increase significantly B cell expansion from CD34+ cord blood mononuclear cell. Conclusion These findings showed that IL-2 and IL-7 significantly increased B cell generation from cord blood CD34+ cells probably this cytokines may be used in ex vivo generation of B cells from cord blood mononuclear cells.
Keywords: B cells, CD34+ cells, Cytokine, Umbilical
Full-Text [PDF 413 kb]   (319 Downloads)    
Type of Study: Research | Subject: Special
Received: 2015/08/22 | Accepted: 2015/08/22 | Published: 2015/08/22
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Aliyari Z, Alami F, Mostafavi T, Taiefi Nasrabadi H, Soleimanirad J, Nozad Charoudeh H. The roles of IL-2, IL-7, and IL15 Ligands in B Cells development. Iran J Ped Hematol Oncol. 2015; 5 (3) :155-160
URL: http://ijpho.ssu.ac.ir/article-1-217-en.html
Volume 5, Number 3 (8-2015) Back to browse issues page
Iranian Journal of Pediatric Hematology and Oncology
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