Volume 6, Issue 3 (9-2016)                   Iran J Ped Hematol Oncol 2016, 6(3): 172-181 | Back to browse issues page

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Eskandari F, Pourkarim H, Sahmani M, Goudarzi M, Mobarra N, Dehghanifard A, et al . Gene Expression and Promoter Methylation Status of VHL, Runx-3, E-cadherin, P15 and P16 Genes During EPO-Mediated Erythroid Differentiation of CD34+ Hematopoietic Stem Cells . Iran J Ped Hematol Oncol 2016; 6 (3) :172-181
URL: http://ijpho.ssu.ac.ir/article-1-264-en.html
Gene Expression and Promoter Methylation Status of VHL, Runx-3, E-cadherin, P15 and P16 Genes During EPO-Mediated Erythroid Differentiation of CD34+ Hematopoietic Stem Cells
Fatemeh Eskandari , Hoda Pourkarim , Mehdi Sahmani , Mehdi Goudarzi , Naser Mobarra , Ali Dehghanifard , Gholamreza Khamisipour , Nasim Kalantari , Hanieh Rahmani , Mehdi Azad *
Department of Medical laboratory sciences, Faculty of Allied Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
Abstract:   (7646 Views)

Background: VHL (von Hippel-Lindau), Runx-3 (Runt-related transcription factor 3), E-cadherin (Epithelial cadherin), P15 (INK4a, cyclin dependent kinase inhibitor), and P16 (INK4b) genes are essential in hematopoiesis. The aim of this study was to explore the correlation between gene expression and promoter methylation in CD34+ stem cells before and after differentiation to erythroid lineage.

Materials and Methods: CD34+ hematopoietic stem cells were separated from umbilical cord blood using MidiMacs (positive selection) system. Expanded CD34+ stem cells were differentiated into erythroid lineage with human recombinant erythropoietin (EPO). DNA extraction was done by QIAamp DNA Mini Kit. RNA was extracted using RNase Mini plus Kit. MSP (Methylation specific PCR) technique was done for methylation assay. Methylation status and expression assay was done for VHL, Runx-3, E-cadherin, P15, and P16 genes  on both CD34+ stem cells and differentiated erythroid cells.

Results: The results showed that, before differentiation, P15 had comparative methylation pattern and average expression and it remained unchanged after differentiation (p=0.01). concerning P16, results revealed no methylation pattern and complete expression in absence of EPO and with EPO it changed to comparative status (p=0.01). E-cad and Runx-3 genes had relative methylation pattern and fully expression before and after differentiation but their expression after that, was increased and decreased  Respectively (p=0.04). VHL gene had no significant methylation status before or after differentiation and its expression was complete (p=0.01).

Conclusion: The obtained results indicated that promoter methylation of P15, P16, VHL, Runx3 and E-cad was one of the definitive expression control mechanism of these genes.

Keywords: Differentiation, EPO, Hematopoietic Stem Cells
Full-Text [PDF 372 kb]   (1676 Downloads)    
Type of Study: Research | Subject: Heart
Received: 2015/09/15 | Accepted: 2016/06/12 | Published: 2016/09/3
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