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Volume 12, Issue 3 (7-2022)
Iran J Ped Hematol Oncol 2022, 12(3): 152-163 |
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Mortazavie F, Taheri S, Tandel P, Zare F, Tamaddon G. The effect of Ganoderic Acid A on miR-17-5p and miR-181b expression level and apoptosis induction in human leukemia Nalm-6 cells. Iran J Ped Hematol Oncol 2022; 12 (3) :152-163
URL: http://ijpho.ssu.ac.ir/article-1-670-en.html
URL: http://ijpho.ssu.ac.ir/article-1-670-en.html
Diagnostic Laboratory Sciences and Technology Research Center, Paramedical School, Shiraz, University of Medical Sciences, Shiraz, Iran
Abstract: (1073 Views)
Background: In various cancers, Ganoderic Acid A (GAA), an active triterpenoid derived from Ganoderma lucidum, has been proved to show potent anti-tumor effects. However, the possible impacts of GAA on the human leukemia cell line (Nalm-6) are not fully elucidated. Therefore, this research aimed to study the antineoplastic effect of GAA on Nalm-6 cells.
Materials and Methods: In this laboratory trial study, Nalm6 cells were cultured in vitro and treated with different doses of GAA (25, 50, 100, 200, and 400 μg/mL) for 24, 48, and 72 hours. The optimal treatment concentration of GAA was determined by the MTT assay. Flow cytometry was used to determine the death of Nalm-6 cells caused by GAA treatment by utilizing FITC-conjugated propidium iodide (PI) and annexin V staining. After incubation, the expression levels of miR-17-5p and miR-181b were monitored using real-time polymerase chain reaction (PCR).
Results: Based on the half-maximal inhibitory concentration (IC50) measurements of the MTT assay, the optimal treatment concentration of GAA was 140 μg/mL (in a dose and time-dependent manner, p<0.0001). The GAA treatment was selectively toxic to the leukemia Nalm-6 cells and could remarkably induce cell apoptosis (p<0.0001). Besides, GAA downregulated the expression of miR-17-5p and miR-181b in the Nalm-6 cells compared with the untreated cells (P=0.0067 and P=0.0014, respectively).
Conclusions: Based on the present findings, GAA merits further investigation as a promising natural reagent for treating hematologic malignancies.
Materials and Methods: In this laboratory trial study, Nalm6 cells were cultured in vitro and treated with different doses of GAA (25, 50, 100, 200, and 400 μg/mL) for 24, 48, and 72 hours. The optimal treatment concentration of GAA was determined by the MTT assay. Flow cytometry was used to determine the death of Nalm-6 cells caused by GAA treatment by utilizing FITC-conjugated propidium iodide (PI) and annexin V staining. After incubation, the expression levels of miR-17-5p and miR-181b were monitored using real-time polymerase chain reaction (PCR).
Results: Based on the half-maximal inhibitory concentration (IC50) measurements of the MTT assay, the optimal treatment concentration of GAA was 140 μg/mL (in a dose and time-dependent manner, p<0.0001). The GAA treatment was selectively toxic to the leukemia Nalm-6 cells and could remarkably induce cell apoptosis (p<0.0001). Besides, GAA downregulated the expression of miR-17-5p and miR-181b in the Nalm-6 cells compared with the untreated cells (P=0.0067 and P=0.0014, respectively).
Conclusions: Based on the present findings, GAA merits further investigation as a promising natural reagent for treating hematologic malignancies.
Type of Study: Research |
Subject:
Heart
Received: 2021/09/27 | Accepted: 2022/05/11 | Published: 2022/07/19
Received: 2021/09/27 | Accepted: 2022/05/11 | Published: 2022/07/19
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