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Volume 13, Issue 4 (10-2023)
Iran J Ped Hematol Oncol 2023, 13(4): 241-250 |
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Elmi A, Atashi A, Gheibi N, Amiri S, Ajami M, Ajami M, et al . Parvovirus B19 Infection May Potentially Determine the Fate of Hematopoiesis by Altering the Human Bone Marrow Mesenchymal Stem Cells Differentiation. Iran J Ped Hematol Oncol 2023; 13 (4) :241-250
URL: http://ijpho.ssu.ac.ir/article-1-683-en.html
URL: http://ijpho.ssu.ac.ir/article-1-683-en.html
Azin Elmi 
, Amir Atashi , Nematollah Gheibi , Shahin Amiri , Monireh Ajami , Mansoureh Ajami , Razieh Mohammadihaji , Naeimeh Khodabandeloo , Mehdi Azad *
, Amir Atashi , Nematollah Gheibi , Shahin Amiri , Monireh Ajami , Mansoureh Ajami , Razieh Mohammadihaji , Naeimeh Khodabandeloo , Mehdi Azad *
Department of Medical Laboratory Sciences, Faculty of Allied Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.
Abstract: (139 Views)
Background: Human bone marrow mesenchymal stem cells (hBM-MSCs), as supporters for hematopoiesis, differentiate into osteoblasts and adipocytes. Studies showed that infection of hBM-MSCs by Parvovirus B19 (B19V) can affect the differentiation capability of hBM-MSCs. This study aims to evaluate B19V effects on the differentiation of hBM-MSCs.
Materials and Methods: In this experimental study hBM-MSCs were cultured up to passage 3. Nucleofection was subsequently employed to deliver a plasmid containing the B19V genome into the cells. The transfected cells were then differentiated into osteoblast and adipocyte lineages. qRT-PCR was then performed to analyze the differentiation 14 days after transfection.
Results: On the 14th day after induction the findings demonstrated a significant increase in adipocyte-specific (PPARγ and LPL) gene expression compared to the control group (p<0.05) and a slight but not statistically significant decrease in the expression of the osteocyte-specific genes (RUNX2 and osteocalcin) (p>0.05).
Conclusion: The results suggest that B19V infection can promote the differentiation of hBM-MSCs towards adipocytes and affect the bone marrow microenvironment as well as hematopoiesis.
Materials and Methods: In this experimental study hBM-MSCs were cultured up to passage 3. Nucleofection was subsequently employed to deliver a plasmid containing the B19V genome into the cells. The transfected cells were then differentiated into osteoblast and adipocyte lineages. qRT-PCR was then performed to analyze the differentiation 14 days after transfection.
Results: On the 14th day after induction the findings demonstrated a significant increase in adipocyte-specific (PPARγ and LPL) gene expression compared to the control group (p<0.05) and a slight but not statistically significant decrease in the expression of the osteocyte-specific genes (RUNX2 and osteocalcin) (p>0.05).
Conclusion: The results suggest that B19V infection can promote the differentiation of hBM-MSCs towards adipocytes and affect the bone marrow microenvironment as well as hematopoiesis.
Type of Study: Research |
Subject:
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Received: 2021/11/12 | Accepted: 2023/09/9 | Published: 2023/10/18
Received: 2021/11/12 | Accepted: 2023/09/9 | Published: 2023/10/18
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